The Inhibitory Ability of Endophytic Bacteria Bacillus sp. BTH-22 and BTH-31a to the Growth of Xanthomonas oryzae in Vitro
DOI:
https://doi.org/10.11594/nstp.2025.4914Keywords:
Xanthomonas oryzae, endophytic bacteria, biological control agentsAbstract
Xanthomonas oryzae (Xoo) is an important pathogenic bacteria in rice plants that causes leaf blight disease. Infection by Xoo disrupts photosynthesis activity and the grain-filling process, in addition, this disease reduces crop yields by 15-80%. Control in the field is generally carried out using bactericides and if carried out continuously it will be dangerous for humans and the environment, so biological control using endophytic bacteria is needed. Endophytic bacteria are microorganisms that have a mutualistic symbiosis with the tissue of eggplant (Solanum melongena) and can be isolated from the plant tissue. Endophytic bacteria that have been successfully isolated are Bacillus sp. Bth-22 and Bth-31a. The purpose of the study was to prove the inhibitory ability of Bacillus sp. Bth-22 and Bth-31a to the growth of Xoo bacteria on Nutrient Agar (NA) media in the laboratory. The study was conducted using a Completely Randomized Design with 3 treatments, namely sterile water (B0), Bacillus sp. Bth-22, (B1) and Bth-31a (B2) with 6 replications. The study was conducted at the Plant Health Laboratory of the Faculty of Agriculture, UPN “Veteran” East Java, located at 7°9?- 7°21? LS and 112° 36? – 112° 54? BT. Data were analyzed using variance (ANOVA) at a 5% error rate. If different results were obtained, further testing was carried out using DMRT (Duncan Multiple Range Test) at a 5% level. Data analysis was performed using IBM SPSS Statistics 24 software. The results of the study showed that inhibition of Xoo growth was Bacillus sp. Bth-22 (B1) 3.9 cm and Bth-31a (B2) 9.3 cm, the inhibition mechanism is antibiosis as a bacteriostatic.
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