The Inhibitory Ability of Endophytic Bacteria Bacillus sp. BTH-22 and BTH-31a to the Growth of Xanthomonas oryzae in Vitro
DOI:
https://doi.org/10.11594/nstp.2025.4914Keywords:
Xanthomonas oryzae, endophytic bacteria, biological control agentsAbstract
Xanthomonas oryzae (Xoo) is an important pathogenic bacteria in rice plants that causes leaf blight disease. Infection by Xoo disrupts photosynthesis activity and the grain-filling process, in addition, this disease reduces crop yields by 15-80%. Control in the field is generally carried out using bactericides and if carried out continuously it will be dangerous for humans and the environment, so biological control using endophytic bacteria is needed. Endophytic bacteria are microorganisms that have a mutualistic symbiosis with the tissue of eggplant (Solanum melongena) and can be isolated from the plant tissue. Endophytic bacteria that have been successfully isolated are Bacillus sp. Bth-22 and Bth-31a. The purpose of the study was to prove the inhibitory ability of Bacillus sp. Bth-22 and Bth-31a to the growth of Xoo bacteria on Nutrient Agar (NA) media in the laboratory. The study was conducted using a Completely Randomized Design with 3 treatments, namely sterile water (B0), Bacillus sp. Bth-22, (B1) and Bth-31a (B2) with 6 replications. The study was conducted at the Plant Health Laboratory of the Faculty of Agriculture, UPN “Veteran” East Java, located at 7°9?- 7°21? LS and 112° 36? – 112° 54? BT. Data were analyzed using variance (ANOVA) at a 5% error rate. If different results were obtained, further testing was carried out using DMRT (Duncan Multiple Range Test) at a 5% level. Data analysis was performed using IBM SPSS Statistics 24 software. The results of the study showed that inhibition of Xoo growth was Bacillus sp. Bth-22 (B1) 3.9 cm and Bth-31a (B2) 9.3 cm, the inhibition mechanism is antibiosis as a bacteriostatic.
Downloads
References
Akrom, A. A., Purnawati, A., & Prasetyowati, E. T. (2024). Potensi bioenkapsulasi bakteri endofit Bacillus sp. sebagai biocontrol busuk batang fusarium pada tanaman jagung. Journal Agroekotek, 16(2), 1-18.
Djatmiko, H. A., Arwiyanto, T., Hadisutrisno, B., & Sunarminto, B. H. (2007). Potensi tiga genus bakteri dari tiga rizosfer tanaman sebagai agensia pengendali hayati penyakit lincat. Jurnal Ilmu-ilmu Pertanian Indonesia, 9(1), 40-47.
Fiyaz, R. A., Shivani, D., Chaithanya, K., Mounika, K., Chiranjeevi, M., Laha, G. S., Viraktamath B. C., Rao, L.V.S., & Sundaram, R.M. (2022). Genetic improvement of rice for bacterial blight resistance: Present status and future prospects. Rice Sci. 29(2), 118-132. doi: 10.1016/j.rsci.2021.08.002.
Hersaputri, S. A. (2023). Aplikasi agensia hayati untuk menghambat penyakit hawar daun bakteri dan meningkatkan pertumbuhan tanaman padi (Oryza sativa L.). Skripsi. Universitas Lampung. Lampung.
Kim, S. M., & Reinke, R. F. (2019). A novel resistance gene for bacterial blight in rice, Xa43(t) identified by GWAS, confirmed by QTL mapping using a biparental population. PloS One, 14(2), e0211775. doi: 10.1371/journal.pone.0211775.
Lv, J., Da, R., Cheng, Y. et al. (2020). Mechanism of antibacterial activity of Bacillus amyloliquefaciens C-1 lipopeptide toward anaerobic clostridium difficile. Bio Med Research International, 3, 2020:3104613.
Magvirah, T., Marwati, & Ardhani, F. (2019). Uji daya hambat bakteri Staphylococcus aureus menggunakan ekstrak daun tahongai (Kleinhovia hospita L.). Journal Peternakan Lingkungan Tropis, 2(2), 41–50.
Purnawati, A. (2013). Efek mikroba endofit terhadap Ralstonia solanacearum Penyebab Layu pada Tanaman Tomat. Disertasi. Universitas Brawijaya. Malang.
Purnawati, A., Triwahyu, P. E., & Fari, A. H. (2024). Potential of encapsulation Bacillus cereus BTH-22 againts bacterial wilt disease on eggplant. Journal Bioteknologi dan biosains Indonesia, 11(1), 155-161.
Sayekti, N. A., Purnawati, A., & Lestari, S. R. (2024). Characterization of bacteria causing leaf blight disease in rice plants in Sidoarjo. Journal of Applied Plant Technology (JAPT), 3(2), 130-136. https://doi.org/10. /xwdype52
Senol, M., Nadaroglu, H., Dikbas, N., & Kotan, R. (2014). Purification of chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum. Annals of Clinical Microbiology and Antimicrobials, 13(35), 1-7.
Sudir, & Yuliani, D. (2016). Composition and distribution of Xanthomonas oryzae pv. oryzae pathotypes, the pathogen of rice bacterial leaf blight in Indonesia. Agrivita, 38(2), 174-185.
Yasmin, S., Hafeez, F. Y., Mirza M. S., Rasul, M., Arshad, H. M. I., Zubair, M., & Iqbal, M. (2017). Biocontrol of bacterial leaf blight of rice and profiling of secondary metabolites produced by rhizospheric Pseudomonas aeruginosa BRp3. Front Microbiol., 8, 1895. doi: 10.3389/fmicb.2017.01895.
Downloads
Published
Conference Proceedings Volume
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this proceedings agree to the following terms:
Authors retain copyright and grant the Nusantara Science and Technology Proceedings right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this proceeding.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the proceedings published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this proceeding.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See the Effect of Open Access).
How to Cite
Share
