Chili Plant Growth Promoted by Bio-Encapsulated Bacillus sp. Biological Control Agents in Different Coating Materials

Authors

  • Yenny Wuryandari Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia
  • Penta Suryaminarsih Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia
  • Safira Rizka Lestari Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia
  • Siswanto Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia

DOI:

https://doi.org/10.11594/nstp.2025.4759

Keywords:

Bacillus sp., biological control, Fusarium wilt, bio-encapsulation

Abstract

Bacillus sp. is a biological control agent widely used to enhance plant growth and control diseases, especially in suppressing Fusarium wilt pathogens. The effectiveness of Bacillus species in controlling diseases varies, with isolates Bcz20, Bcz14, and Bcz16 showing the highest inhibition. Fusarium sp. a fungal pathogen, isolated from infected chili plants, produces characteristic crescent-shaped spores. For bio-encapsulation, various coating materials such as sodium alginate, carrageenan, and chitosan were used. Sodium alginate proved to be the most compatible coating material, effectively maintaining the Bacillus population over time. The encapsulation efficiency was tested through a detailed process, which revealed Bacillus isolate Bcz20 as the most viable among the various coating materials, especially sodium alginate. In VIVO tests on chili plants showed that bio-encapsulation of Bacillus sp. did not show significant differences in height but showed better root development in the treatment using Bacillus Bcz20 with carrageenan. Bacillus sp. plays an important role in enhancing plant growth and promoting systemic resistance, making it a promising agent for sustainable agriculture.

Downloads

Download data is not yet available.

References

Agrios, G. N. (2005). Plant Pathology (Fifth Edit). Academic Press.

Choudhary, D. K., & Johri, B. N. (2008). Interaction of Bacillus spp. and plants-with special special reference to induced systemic resistance (ISR). Microbiol Res., 164(5), 493-513. doi:10.1016/j.micres.2008.08.007.

Elad, Y., & Freeman, S. (2002). Biological control of fungal plant pathogen. pp. 92- 109. In Kempken (Ed.), The Mycota XI, Agricultural Applications. SpringerVerlag, Berlin.

Gond, S. K., Bergena, M. S., Torresa, M. S., & White, J. F. Jr. (2015). Endophytic Bacillus spp. produce antifungal lipopeptides and induce host defence gene expression in maize. Microbiological Research, 172, 79–87.

Junaid, J. M., Dar, N. A., Bhat, T. A., Bhat, A. H., & Bhat, M. A. (2013). Commercial biocontrol agents and their mechanism of action in the management of plant pathogens. Int. J. Modern Plant & Anim. Sci. 1(2), 39-57.

Khan, N. H., Korber, D. R., Low, N. H., & Nickerson, M. T. (2013). Development of extrusion-based legume protein isolate–alginate capsules for the protection and delivery of the acid-sensitive probiotic, Bifidobacterium adolescentis. Food Research International, 54(1), 730-737.

Malfanova, N.V. (2013). Endophytic bacteria with plant growth promoting and biocontrol abilities. Leiden University Repository, 15-37.

Mugiastuti, E., Manan, A., Rahayuniati, R. F., & Soesanto, L. (2019). Aplikasi Bacillus spp. untuk mengendalikan penyakit layu fusarium pada tanaman tomat. Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto Jl. dr. Soeparno, Karangwangkal, Purwokerto, Jawa Tengah.

Morales-Rodriguez, I., Yanez-Morales, M. J., Silva-Rojas, H. V., de los Santos, G. G., Guzman-de-Munkvold, G. P. (2003). Epidemiology of Fusarium diseases and their mycotoxins in maize ears. European Journal of Plant Pathology, 109, 705-713.

Pour, M. M., Riseh, R. S., Ranjbar-Karimi, R., Hassanisaadi, M., Rahdar, A., & Baino, F. (2022). Microencapsulation of Bacillus velezensis using alginate-gum polymers enriched with TiO2 and SiO2 Nanoparticles. Micromachines, 13(9), 1423.

Prakash, J., & Arora, N. K. (2019). Phosphate-solubilizing Bacillus sp. enhances growth, phosphorus uptake and oil yield of Mentha arvensis L. 3 Biotech., 9(4), 126. doi: 10.1007/s13205-019-1660-5.

Prihatiningsih, N., Arwiyanto, T., Hadisutrisno, B., & Widada, J. (2015). Mekanisme Antibiosis Bacillus Subtilis B315 untuk pengendalian penyakit layu bakteri kentang. Fakultas Pertanian Unsoed Purwokerto Kampus Karangwangkal. Yogyakarta.

Radhakrishnan, R., & Lee, I. (2016). Gibberellins producing Bacillus methylotrophicus KE2 supports plant growth and enhances nutritional metabolites and food values of lettuce. Plant Physiology and Biochemistry, 109, 181-189.

Saad, M. M., Abo-Koura, H. A., Bishara, M. M., & Gomaa, I. M. (2020). Microencapsulation: Toward the reduction of the salinity stress effect on wheat plants using NPK rhizobacteria. Biotechnology Journal International, 23(4), 1–18.

Suryani, N., Betha, O. S., & Mawaddana, Q. (2019). Uji viabilitas mikroenkapsulasi LactoBacillus casei menggunakan matrik natrium alginat. Jurnal Farmasi Lampung, 8(1), 1–8.

Szczech, M., & Maciorowski, R. (2016). Microencapsulation technique with organic additives for biocontrol agents. Journal of Horticultural Research, 24(1), 111–122.

Uyen, N. T. T., Hamid, Z. A. A., Tram, N. X. T., & Ahmad, N. (2020). Fabrication of alginate microspheres for drug delivery: A review. International Journal of Biological Macromolecules, 153, 1035–1046.

Wuryandari, Y, Lestari, S.R., Mahendra, R. 2022. Kemampuan antagonistik bakteri Bacillus Spp. terhadap patogen Fusarium sp. penyebab penyakit layu tanaman cabai (Capsicum Annum L.) dan mekanisme kerjanya. Laporan Akhir. Riset Pui-PT Pusat Inovasi Teknologi Tepat Guna Pangan Dataran Rendah Dan Pesisir

Zhang, S., Raza, W., Yang, X., Hu, J., Huang, Q., Xu, Y., Liu, X., Ran, W., & Shen, Q. (2008). Control of Fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer. Biol Fertil Soils, 44, 1073–1080.

Downloads

Published

15-05-2025

Conference Proceedings Volume

Section

Articles

How to Cite

Wuryandari, Y., Suryaminarsih, P., Lestari, S. R., & Siswanto. (2025). Chili Plant Growth Promoted by Bio-Encapsulated Bacillus sp. Biological Control Agents in Different Coating Materials. Nusantara Science and Technology Proceedings, 2024(47), 392-399. https://doi.org/10.11594/nstp.2025.4759

Similar Articles

61-70 of 426

You may also start an advanced similarity search for this article.