Detection of Micronucleus, Nucleoplasmic Bridges, and Nuclear Buds Frequency in Peripheral Blood Lymphocytes of Cancer Patient Post-Radiation Fractionated
DOI:
https://doi.org/10.11594/nstp.2021.0707Keywords:
Micronucleus, nucleoplasmic bridge, nuclear buds, radiation, cancer patientsAbstract
Simple measurement of cytogenetic damage would be of great value for studying genetic risk factors, especially in radiotherapy for cancer patients. One cytogenetic technique that is easy and simple to quantify the damage caused by radiation exposure in cultured human lymphocytes is the micronucleus (MN). This research was conducted to study the induction of micronucleus (MN), Nucleoplasmic Bridge (NPB), and Nuclear Buds (NBUDs) in cancer patients after administration of fractionated radiation exposure total of 20 Gy. Peripheral blood lymphocyte samples obtained from eleven cancer patients as the study group and eleven from the healthy people as the control group were assessed. Both samples were then cultured and added cytochalasin-B to arrest cells during the cytokinesis stage. Its characteristics were observed in binucleated cells (BNC) with cytochalasin blocked micronuclei (CBMN) assay procedure. The number of MN, NPB, and NBUDs was evaluated per 1000 BNC for both the study group and control. The results showed that there was a statistically signif- icant difference (P <0.05) between the frequency of MN in the study group (82.18±39.93) compared to controls (13.18±4.94). Besides, the number of NPB and NBUD in the study group is relatively low. In conclusion, the iden- tification of MN formation in peripheral blood lymphocytes of post-radiation cancer patients has other molecular mechanisms such as NPB and NBUD. Also, demographic factors such as age can influence the appearance of MN, NPB, and NBUDs.
Downloads
Published
Issue
Section
License
Authors who publish with this proceedings agree to the following terms:
Authors retain copyright and grant the Nusantara Science and Technology Proceedings right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this proceeding.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the proceedings published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this proceeding.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See the Effect of Open Access).
